Part:BBa_K4367006

ZF_TM_Cub

The ZF transmembrane cub complex protein(ZM_TM_Cub) is a transmembrane protein with a complementary complex, the Zinc finger transmembrane NubG complex (ZF_TM_NubG), that is designed to detect a specific DNA sequence. It is based on a membrane based yeast two hybrid system. When the ZF domains in the two complexes bind to their target DNA sequences on the same DNA molecule, the nub and cub domains are brought to close proximity activating the split ubiquitin, which releases the transcription factor.

ZF_TM_Cub protein contains multiple domains: signaling sequence domain, the Zinc finger domain, extracellular linker domain, transmembrane domain, B2 linker domain, cub domain, and transcription factor domain. The zinc finger domain in this complex binds to the sequence: TAGCAGCTG. The cub domain is a part of the split ubiquitin used in membrane yeast two hybrid system. The membrane yeast two hybrid system is normally used to test for protein interaction between membrane proteins. With the Zinc Finger nub and cub complexes, the binding to the target DNA sequence corresponds to a protein-protein interaction.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1615
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1615
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1615
  Illegal BglII site found at 1545
  Illegal BamHI site found at 1202
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1615
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1615
  
• 1000
  COMPATIBLE WITH RFC[1000]